Garcia de la Torre, J.; Carrasco, B. Walkenhorst, S.; Olivier, R. (2003). Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. For data requests, please contact the authors. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Biomaterials. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Progr. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. 4h after microporation, the sizes of the micropore decreased slightly. There is a deviation from linearity after 580kDa [69, 70]. J. Braz. Int J Food Sci Nutr. 7 8 Macromolecules. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Structure Properties of Dextran. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. The skin poration was performed using the microporation device (LG electronics, Korea). Journal of Applied Polymer Science, 27, 795-810. Huggins, Maurice L. (1942). Eng. J Appl Toxicol. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Front Microbiol. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Walkenhorst, S.; Olivier, R. (2002). The Mark-Houwink a constant is close to 0.5 in poor solvents. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Carbohydrate Research, 340, 811-826. TEWL measurements were performed before and 30min after microporation. Chen Rong Huei; Min Lang Tsai (1998). Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish ; Aberle, T; Burchard, W. (2000). On the molecular weight distribution of dextran T-500. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Nat Biotechnol. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Biotechnology Progress, 19, 544-548. Carbohydrate Polymers, 38, 267-271. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Guner, A.; Kibarer, G. (2001). In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Rheology of dextran solutions. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Progress in Biophysical Molecules Biological, 68, 207-262. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Table 2 compares the properties of nanoparticles coated with one or other polymer. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Fixable: Fixable via Lysine. ; Fran, H.P. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. Carbohydrate Polymers 95 360-365. 2011;5(2):95132. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. (1987). Soc., 21, 1, 78-86. (2013). Human skins (Full-thickness human skin) were supplied by HansBiomed. Carbohydrate Polymers, 78, 902-907. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Microporation techniques for enhanced delivery of therapeutic agents. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Not for use in diagnostic procedures. Journal of Applied Polymer Science, 13, 2031-2032. After 24h, the cumulative permeation of FITC-dextran M.W. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Solvent effects on polysaccharide conformation. Manage cookies/Do not sell my data we use in the preference centre. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Article Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Carbohydrate Polymers 87, 405-410. Dextran. Amersham Biosciences, AA Edition, www.amershambiosciences.com. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. 2008;28(5):64557. PMC 2013, Article ID 360239, pp. Harding, Stephen E. (2005). We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. The solution was measured using an Anton Paar DMA35N densitometer. (2007). The Mark-Houwink parameters and the effect of temperature on them are specifically studied. (2007). Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Trop J Pharm Res. Gold MH, Biron JA. 2012;29(7):201729. American Journal of Food Science and Technology 2013, 1(3), 60-66. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Springer Nature. government site. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Anal. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Durand, A. 20K groups (n=3, *: p<0.05, **: p<0.01). The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Search Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Colloid Polym. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. 2023 BioMed Central Ltd unless otherwise stated. 2013;3(5):3588605. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Physicochemical studies of oligodextran. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Macromolecules, 33, 5730-5739. Viscosity of Liquids. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Expert Rev Med Devices. National Library of Medicine Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Dependence on Concentration. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Terms and Conditions, Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). (2007). Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). (1958). Gekko, K. (1971). WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. 151-166. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Carbohydr Polym. eCollection 2018 Feb. Molecules. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. On single point determination of intrinsic viscosity. ; Patel, T.R. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Proc. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Kasaai, Mohammad R. (2012). Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Hrabchak C, Flynn L, Woodhouse KA. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Intrinsic viscosity molecular weight relationship. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. ; Picout, D.R. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. An undergraduate physical chemistry experiment. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Hydrodynamic Properties of Tragacanthin. Carbohydrate Polymers 72, 356-360. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. HSE and DK helped to porate the skin and to measure TEWL. The effect of branching is best recognized from the molecular weight dependencies of []. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. CAS Format). The mechanism of the dextran-induced red blood cell Aggregation. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. 2012;87(6):1728. Carbohydrate Polymers, 74, 372-378. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Masuelli, Martin Alberto. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Masuelli, M. A. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. The human and pig skin permeability of FITC-dextran (M.W. Journal of Physical Chemistry, 58(11), 953-957. eCollection 2015. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. 2012;3(1):208. European Polymer Journal, 37, 619-622. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. 2000;3(9):31826. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Pathan IB, Setty CM. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Bahary, W.S. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. dextran hydrodynamic radius. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Polysaccharide hydrogels for modified release formulations. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Epub 2021 Feb 9. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. Charge: Anionic. osmotic pressure or light scattering). 2003;89(2):31120. Intrinsic viscosity of bovine serum albumin conformers. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. J. Braz. Biomacromolecules 8, 2464-2475. With FITC-dextran 20 K, cumulative WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. (1954). J. The Viscosity of Dilute Solutions of Long-Chain Molecules. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Differences were considered statistically significant at p <0.05. Prausnitz MR, Langer R. Transdermal drug delivery. 2003;10:33847. 4K and M.W. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. 2010;4(1):117. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Sachdeva V, Banga AK. @ Nilimesh See attached. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Journal of American Chemical Society, 64(11), 2716-2718. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. He, L.; Niemeyer, B. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Biophysical Journal, 27, 177-181. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. (1999). The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. A single frequency laser is directed to the sample contained in a cuvette. Microporation was performed 2 times on each sample. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea).